Application of Glycosyltransferases to Functionalise Protocells
This research relies on the use of glycosyltransferases (GTs) to expand the tool box of synthetic glycobiology methods. GTs are the key enzymes that synthesize the complex glycans that are found on glycoconjugates (i.e. glycolipids and glycoproteins). Therefore, they present a great interest in the context of the SynBioCarb project for building a sophisticated glyco-landscape on the surface of artificial cells or tissues with applications in tissue engineering, drug delivery and diagnostics. This opens the possibility to add one or several sugars at specific position(s) of a glycan or to introduce unnatural sugars that could serve for biorthogonal chemistry. A small set of GTs, with specificities related to glycans selected by the consortium, will be produced in recombinant forms and functionally characterised and/or engineered to be adapted to the needs of the project.
CERMAV – CNRS
Supervisor Prof Christelle Breton